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US 2016/0237392 A1Published application
Apparatus and method for continuous cell culture
AssigneeMedikan Co., Ltd.
InventorHee Young LEE
Life of this patent
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Abstract
Disclosed is a method of continuously culturing cells, including culturing the cells by injecting a culture medium into an internal compartment of a sealed culture vessel and then inoculating the culture medium with the cells, detaching the adhered cells when the density of the cells cultured in the compartment of the culture vessel is a reference value or more, and obtaining the cells detached from the culture vessel, which is maintained in a sealed state.
Claims32
A device for continuously culturing cells, comprising:
- a culture vessel having a compartment that is selectively determined to be sealed,
- wherein a culture medium is injected into the compartment and inoculated with the cells and the cells are detached and obtained while the compartment is sealed, and the cells are cultured while the compartment is open.
A method of continuously culturing cells, comprising:
- culturing the cells by injecting a culture medium into an internal compartment of a sealed culture vessel and then inoculating the culture medium with the cells;
- detaching the adhered cells when a density of the cells cultured in the compartment of the culture vessel is a reference value or more;
- and
- obtaining the cells detached from the culture vessel, which is maintained in a sealed state.
1 more element
Dependencies are inferred from the claim text.