Tissue extracellular matrix particles and applications
AssigneeThe Johns Hopkins University
InventorsVincent Beachley, Matt Gibson, Jennifer Elisseeff
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Abstract
An apparatus in the form of a chip is provided wherein the apparatus is prepared with decellularized extracellular matrix from various tissues and can be used to investigate the cellular interactions between the ECM and the various cell types. Three dimensional culture methods for investigating decellularized extracellular matrix from various tissues and interactions with various mammalian cell types are also provided. Methods of use of cells grown using the apparatus and methods disclosed are also provided.
Claims19
An apparatus for culturing cells comprising:
- a first functionalized substrate having at least one functionalized surface;
- a gel pad disposed on the functionalized surface of the first functionalized substrate;
- an array comprising a plurality of discrete layers of collagen positioned on top of the gel pad, each having a defined area and being substantially aligned with one another and defining a space between one another;
- and
- a layer of decellularized extracellular matrix particles (DECM) positioned on top of at least one or more of the discrete layers of collagen, wherein the DECM is capable of supporting cellular growth.
A process for forming spheroid aggregates of mammalian stem cells and decellularized extracellular matrix (DECM) particles comprising:
- a) preparing a suspension of DECM particles in a suitable growth media;
- b) preparing a suspension of mammalian stem cells in the same suitable growth media;
- c) preparing a mixture of a) and b) at a ratio in a range of 10:1 to 1:10 v/v DECM particle solution:mammalian cells;
- d) suspending the mixture of c) in a hanging drop culture for a period of between 2 days and 7 days;
- e) replacing the growth media with a suitable induction media;
- and
- f) allowing the culture to grow for period of time sufficient to produce a spheroid aggregate comprising mammalian stem cells and DECM.
A method of implanting spheroid aggregates of mammalian stem cells and decellularized extracellular matrix (DECM) particles in a subject comprising:
- a) identifying a subject in need of spheroid aggregates of mammalian stem cells and decellularized extracellular matrix (DECM) particles;
- b) identifying a site in the subject in need of implantation of spheroid aggregates of mammalian stem cells and decellularized extracellular matrix (DECM) particles;
- and
- c) implanting the spheroid aggregates of mammalian stem cells and decellularized extracellular matrix (DECM) particles in the subject at the identified site.
Dependencies are inferred from the claim text.