Anti-epcam antibodies and methods of use

An immunoconjugate comprising an antibody attached to an effector molecule, wherein the antibody comprises a heavy chain having an amino acid sequence selected from SEQ ID NO:

• 1, SEQ ID NO:
• 11, SEQ ID NO:
• 31, and SEQ ID NO:
• 32, and a light chain having an amino acid sequence selected from SEQ ID NO:
• 2 and SEQ ID NO:
• 12.

A use of an immunoconjugate in the treatment of a subject having cancer, the immunoconjugate comprising an antibody attached to an effector molecule, wherein the antibody comprises a heavy chain having an amino acid sequence selected from SEQ ID NO:

• 1, SEQ ID NO:
• 11, SEQ ID NO:
• 31, and SEQ ID NO:
• 32, and a light chain having an amino acid sequence selected from SEQ ID NO:
• 2 and SEQ ID NO:
• 12.

A method of detecting or monitoring cancer in a subject comprising the steps of:

• contacting a test sample taken from the subject with an antibody to form an antibody-antigen complex, wherein the antibody comprises a heavy chain amino acid sequence selected from SEQ ID NO:
• 1, SEQ ID NO:
• 11, SEQ ID NO:
• 31, and SEQ ID NO:
• 32, and a light chain having amino acid sequence selected from SEQ ID NO:
• 2 and SEQ ID NO:
• 12;
• measuring the amount of the antibody-antigen complex in the test sample;
• and
• normalizing the results against a control.

A method of imaging a tumor in a subject, the method comprising:

• administering to the subject an antibody comprising a heavy chain having an amino acid sequence selected from SEQ ID NO:
• 1, SEQ ID NO:
• 11, SEQ ID NO:
• 31, and SEQ ID NO:
• 32, and a light chain having an amino acid sequence selected from SEQ ID NO:
• 2 and SEQ ID NO:
• 12;
• and
• detecting the antibody by in vivo imaging.

A method of treating a subject with cancer, the method comprising:

• administering a therapeutically effective amount of an immunoconjugate comprising an antibody attached to an effector molecule, wherein the antibody comprises a heavy chain having an amino acid sequence selected from SEQ ID NO:
• 1, SEQ ID NO:
• 11, SEQ ID NO:
• 31, and SEQ ID NO:
• 32, and a light chain having an amino acid sequence selected from SEQ ID NO:
• 2 and SEQ ID NO:
• 12.