Anti-hpv e7 antibodies

An in vitro method for detection of an HPV E7 protein from HPV 16 subtypes or HPV 18 or HPV 45, the method comprising:

• i) incubating a biological sample with at least one monoclonal antibody selected from a group consisting of:
• a monoclonal anti-HPV (human papillomavirus) E7 antibody, which is capable of specifically recognising an epitope of the C-terminal region of an HPV 16 subtype E7 protein, wherein the epitope comprises the amino acid sequence set forth in SEQ ID No. 2, 3, 6, or 7, and
• a monoclonal anti-HPV (human papillomavirus) E7 antibody, which is capable of specifically recognising an epitope of the N-terminal region of an HPV 18 E7 protein, wherein the epitope comprises the amino acid sequence set forth in SEQ ID No. 5 or 8;
• and
• ii) measuring and/or detecting the HPV E7 protein in the biological sample by measuring and/or detecting the monoclonal antibody specifically bound to the E7 protein.

An in vitro method for diagnosis of HPV infections, the method comprising:

• a) coating a support with capture antibodies directed against HPV 16 subtype, HPV 18 or HPV 45 E7 proteins;
• b) adding a biological sample to the coated support;
• c) incubating the support with a monoclonal detection antibody, wherein the monoclonal detection antibody is at least one monoclonal antibody selected from a group consisting of:
• a monoclonal anti-HPV (human papillomavirus) E7 antibody, which is capable of specifically recognising an epitope of the C-terminal region of an HPV 16 subtype E7 protein, wherein the epitope comprises the amino acid sequence set forth in SEQ ID No. 2, 3, 6, or 7, and
• a monoclonal anti-HPV (human papillomavirus) E7 antibody, which is capable of specifically recognising an epitope of the N-terminal region of an HPV 18 E7 protein, wherein the epitope comprises the amino acid sequence set forth in SEQ ID No. 5 or 8;
• and
• d) identifying HPV 16 subtype or HPV 18 or HPV 45 E7 protein specifically bound by the capture antibody by detecting the monoclonal detection antibody specifically bound to the HPV 16 subtype or HPV 18 or HPV 45 E7 protein and thereby allowing a diagnosis of a HPV infection.

An in vitro method for diagnosis of HPV infections, the method comprising:

• a) coating a support with a monoclonal capture antibody, wherein the monoclonal capture antibody is at least one monoclonal antibody selected from a group consisting of:
• a monoclonal anti-HPV (human papillomavirus) E7 antibody, which is capable of specifically recognising an epitope of the C-terminal region of an HPV 16 subtype E7 protein, wherein the epitope comprises the amino acid sequence set forth in SEQ ID No. 2, 3, 6, or 7, and a monoclonal anti-HPV (human papillomavirus) E7 antibody, which
• is capable of specifically recognising an epitope of the N-terminal region of an HPV 18 E7 protein, wherein the epitope comprises the amino acid sequence set forth in SEQ ID No. 5 or 8;
• b) adding a biological sample to the coated support;
• c) incubating the support with detection antibodies directed against HPV 16 subtype, HPV 18 E7 or HPV 45 proteins;
• and
• d) identifying HPV 16 subtype or HPV 18 or HPV 45 E7 protein specifically bound by the capture antibody by detecting the monoclonal detection antibody specifically bound to the HPV 16 subtype or HPV 18 or HPV 45 E7 protein and thereby allowing a diagnosis of a HPV infection.