Synthetic Single Domain Antibody
AssigneesInserm (Institut National De La Sante Et De La Recherche Medicale), Institut Curie, Universite Paul Sabatier Toulouse III, Centre National de la Recherche Scientifique (CNRS)
InventorsAurellen Olichon, Sandrine Moutel, Franck Perez
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Abstract
The invention relates to the identification of a highly stable single domain antibody scaffold (hs2d Ab) and its use in generating synthetic single domain antibody library (hs2d Ab-L1). The invention also relates to antigen-binding proteins comprising said stable single domain antibody scaffold and their uses, in particular as therapeutics.
Claims16
A method of making a synthetic single domain antibody library, said method comprising
- i. introducing a diversity of nucleic acids encoding CDR1, CDR2, and CDR3, between the respective framework coding regions of a synthetic single domain antibody to generate nucleic acids encoding a diversity of synthetic single domain antibodies with the same synthetic single domain antibody scaffold amino acid sequence,
- wherein said synthetic single domain antibody scaffold amino acid sequence contains at least the following amino acid residues:
- F37, E44, R45, F47, and;
- at least the following amino acid residues:
- P15, S49, S81, R93, A94, and optionally further comprising the residues Q8, Q108 and T99, wherein the positions of amino acid residues are indicated according to the Kabat nomenclature used for VH amino acid sequence.
An antigen-binding protein, comprising a synthetic single domain antibody of the following formula:
- FR1-CDR1-FR2-CDR2-FR3-CDR3-FR4, wherein said framework regions FR1, FR2, FR3, and FR4 contains at least the following original camelid VHH amino acid residues:
- F37, E44, R45, F47, and;
- at least the following humanized amino acid residues:
- P15, S49, S81, R93, A94.
Dependencies are inferred from the claim text.