Protein affinity purification resins with tagged protein
AssigneePurdue Research Foundation
InventorsTamara Lea Kinzer-Ursem, Chethana Kulkarni, Julia Grace Fraseur
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Abstract
Disclosed herein are protein enrichment substrates and methods for making the same that can be used to enrich proteins of interest. Also disclosed herein are methods for protein purification using these protein enrichment substrates.
Claims25
A protein enrichment substrate prepared by a process comprising incubating a starting substrate with a mixture to produce a functionalized substrate to thereby make the protein enrichment substrate, wherein the mixture comprises a bioorthogonally tagged protein for selective reaction with the substrate.
A method for making an protein enrichment substrate, comprising:
- incubating a substrate with a mixture to produce a functionalized substrate to thereby make the protein enrichment substrate.
A method of protein enrichment, comprising
- incubating a protein enrichment substrate with a buffer to produce an equilibrated mixture of substrate and buffer;
- further incubating the equilibrated substrate with a crude mixture containing a binding protein to produce a second mixture of functionalized substrate and crude mixture to produce a protein bound substrate;
- washing the protein bound substrate to produce a washed substrate;
- and
- eluting the binding protein from the substrate with another buffer to thereby result in an enriched protein.
Dependencies are inferred from the claim text.