Process for purification of neutral human milk oligosaccharide using simulated moving bed chromatography
AssigneeJennewein Biotechnologie GmbH
InventorsStefan JENNEWEIN, Markus HELFRICH
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Abstract
The present application discloses a process for the purification of a neutral human milk oligosaccharide (neutral HMO). The process uses simulated moving bed (SMB) chromatography which allows the continuous purification of large quantities of HMOs with high purity. Contrary to chemical synthesis routes of neutral HMOs, and their subsequent purification, the presented process allows the provision of HMOs free of noxious chemicals, such as e.g. trace amounts of heavy metals or organic solvents. The individual neutral HMO product may be obtained in solid form by spray drying or as a concentrated syrup. The provided neutral HMO is very well-suited for use in food applications.
Claims18
Process for purification of a neutral human milk oligosaccharide in a continuous chromatography from a crude solution comprising the neutral human milk oligosaccharide and contaminants,
- wherein the crude solution comprising the neutral human milk oligosaccharide and contaminants comprises or consists of a solution which is selected from the group consisting of microbial fermentation extract, biocatalysis reaction solution, chemical synthesis solution, and combinations thereof, wherein the purity of the neutral human milk oligosaccharide in the solution is <80%,
- characterized in that the crude solution is applied to at least one purification step using simulated moving bed chromatography, wherein a purified solution comprising the neutral human milk oligosaccharide with a purity of ≧80% is provided.
Dependencies are inferred from the claim text.