Silk/platelet composition and use thereof
AssigneeTufts University
InventorsIsabella Pallotta, Jonathan Kluge, Alessandra Balduini, David L. Kaplan
Life of this patent
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Abstract
The present disclosure relates generally to silk fibroin and unlysed platelet compositions. The compositions are useful for a variety of medical uses such as wound healing or repair and soft tissue repair or augmentation.
Claims37
A composition comprising silk fibroin and unlysed platelets.
A composition for controlled release of a growth factor, the composition comprising silk fibroin and unlysed platelets.
A method for tuning stiffness of a hydrogel made through sonication, the method comprising varying one or more of the following:
- (i) amount of silk in the hydrogel;
- (ii) amount of platelets (e.g., platelet gel) in the hydrogel;
- (iii) ratio of amount of silk to platelets (e.g., platelet gel) in the hydrogel;
- (iv) silk fibroin concentration of the solution used to make the hydrogel;
- (v) molecular weight of the silk in the hydrogel;
- (vi) silk degumming time, e.g., cocoon boiling time;
- (vii) amount of additive in the hydrogel;
- (viii) net negative charge of silk in the hydrogel;
- (ix) conformation of silk in the hydrogel;
- and
- (x) any combination of (i)-(ix).
A method for altering the ionic and/or electrostatic properties of a hydrogel, the method comprising varying the net charge of silk fibroin in the hydrogel, wherein the net charge is varied by (i) modifying the silk fibroin with one or more negatively charged molecules;
- (ii) varying amount of modified silk fibroin;
- (iii) varying the ratio of modified silk fibroin to unmodified silk fibroin;
- or (iv) any combination of (i)-(iii).
A method of controlling degradation of an implant and therefore the growth factor delivery, the method comprising varying one or more of the following:
- (i) amount of silk in the implant;
- (ii) amount of platelets (e.g., platelet gel) in the implant;
- (iii) ratio of amount of silk to platelets (e.g., platelet gel) in the implant;
- (iv) silk fibroin concentration of the solution used to make the implant;
- (v) molecular weight of the silk in the implant;
- (vi) silk degumming time, e.g., cocoon boiling time;
- (vii) amount of additive in the implant;
- (viii) net negative charge of silk fibroin in the implant;
- (ix) conformation of silk in the hydrogel;
- and
- (x) any combination of (i)-(ix).
A method of controlling release of growth factors from an implant, the method comprising varying the net charge of the implant.
A composition comprising silk fibroin and growth factors derived by activated platelets, wherein the composition is substantially free of platelets.
A composition of any claims where the material gives solution stability.
A double-network hydrogel with a first network and a second network, wherein said first network comprises a platelet gel and said second network comprises a silk fibroin gel.
Dependencies are inferred from the claim text.