Back to results
US 2016/0230201 A1Published application
Large scale enzymatic synthesis of oligosaccharides
AssigneeAcademia Sinica
InventorsChi-Huey WONG, Tsung-I TSAI, Chung-Yi WU
Life of this patent
Estimating the term…
Abstract
A novel UDP-Gal regeneration process and its combined use with a galactosyltransferase to add galactose to a suitable acceptor substrate. Also described herein are synthetic methods for generating Globo-series oligosaccharides in large scale, wherein the methods may involve the combination of a glycosyltransferase reaction and a nucleotide sugar regeneration process.
Claims24
1-34. (canceled)
A method for enzymatically synthesizing an oligosaccharide, comprising:
- (i) producing UDP-GalNAc from GalNAc in the presence of a set of UDP-GalNAc regeneration enzymes, wherein the set of UDP-GalNAc regeneration enzymes comprises an N-acetylhexosamine 1-kinase, an N-acetylglucosamine 1-phosphate uridyltransferase, a pyruvate kinase, and optionally, a pyrophosphatase, and
- (ii) converting Gb3-OR1A into Gb4-OR1A in the presence of the UDP-GalNAc and a beta-1,3-N-acetylgalactosaminyltransferase, wherein R1A is hydrogen, substituted or unsubstituted alkyl, substituted or unsubstituted alkenyl, substituted or unsubstituted alkynyl, substituted or unsubstituted carbocyclyl, substituted or unsubstituted heterocyclyl, substituted or unsubstituted aryl, substituted or unsubstituted heteroaryl, or an oxygen protecting group.
57-99. (canceled)
Dependencies are inferred from the claim text.