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OwnerJiangfeng Zhu, Niels Bjerg Jensen +3inventors
InventorsJIANGFENG ZHU, NIELS BJERG JENSEN, XIAO CHEN, JOCHEN FÖRSTER, IRINA BORODINA
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Recombinant microbial cells and methods for producing 5HTP, melatonin and related compounds using such cells are described. More specifically, the recombinant microbial cell may comprise exogenous genes encoding one or more of an L-tryptophan hydroxylase, a 5-hydroxy-L-tryptophan decarboxylyase, a serotonin acetyltransferase, an acetylserotonin O-methyltransferase; and means for providing tetrahydrobiopterin (THB), and can be further genetically modified to enrich one or more of tryptophan, S-adenosyl-L-methinonine and acetyl coenzyme A. Related sequences and vectors for use in preparing such recombinant microbial cells are also described.
A recombinant microbial cell comprising exogenous nucleic acid sequences encoding an L-tryptophan hydroxylase (TPH) (EC 1.14.16.4), a 5-hydroxy-L-tryptophan decarboxylase (DDC) (EC 4.1.1.28), a serotonin acetyltransferase (AANAT) (EC 2.3.1.87 or EC 2.3.1.5), an acetylserotonin O-methyltransferase (ASMT) (EC 2.1.1.4), and enzymes providing at least one pathway for producing tetrahydrobiopterin (THB), wherein the recombinant microbial cell
A recombinant microbial cell comprising exogenous nucleic acid sequences encoding a TPH (EC 1.14.16.4) and enzymes providing at least one pathway for producing THB,
A method of producing melatonin, comprising culturing a recombinant microbial cell in a medium comprising at least one carbon source and, optionally, isolating melatonin, optionally wherein the medium comprises at least 0.1 g/L methionine and/or at least 0.1 g/L SAM, wherein the recombinant microbial cell comprises:
Dependencies are inferred from the claim text.