Rna based method to obtain stably integrated retroviral vectors
AssigneeCellectis
InventorsAndrew SCHARENBERG, Julianne SMITH, Roman GALETTO
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Abstract
The present invention relates to viral transformation method, particularly foamy virus-mediated transformation method. The present invention relates to the transfer of transgene into cells by the safe and efficient transfer of RNA encoding foamy components. The present invention has therefore therapeutic interest, especially in the field of gene therapy.
Claims16
A method for integrating a transgene within the genome of a cell comprising:
- (a) Providing at least one RNA comprising:
- (i) sequence encoding foamy virus Gag protein, (ii) sequence encoding foamy virus Pol protein;
- (iii) Foamy Virus genomic sequence and a transgene;
- (b) Introducing said RNA into a cell.
An isolated cell comprising RNAs encoding foamy virus Gag and Pol proteins and foamy virus genomic RNA.
A kit comprising one or more RNA(s) comprising a sequence encoding foamy virus Gag protein, a sequence encoding a foamy virus Pol protein, a foamy virus genomic sequence and a transgene, optionally a sequence encoding a fusion deficient Env protein.
Dependencies are inferred from the claim text.