Rnai for the control of phytopathogenic fungi and oomycetes by inhibiting the expression of cyp51 genes
AssigneeBASF SE
InventorKarl-Heinz Kogel
Life of this patent
Estimating the term…
Abstract
The present invention relates to dsRNA molecules for inhibiting the expression of CYP51 gene(s) from a fungal or oomycete phytopathogen and the use of such inhibitory dsRNA molecules for controlling phytopathogenic fungi and/or oomycetes. The present invention further relates to DNA sequences providing a transcriptional template for inhibitory dsRNA molecules or antisense RNA; and phytopathogen-tolerant transgenic plants with such DNA sequences.
Claims25
A dsRNA molecule capable of inhibiting the expression of the CYP51A gene, the CYP51B gene and the CYP51C gene from a phytopathogen of the genus Fusarium, the dsRNA comprising:
- i) a sense sequence being at least 70% identical to at least 20 contiguous nucleotides of the coding sequence of the CYP51A gene;
- ii) a sense sequence being at least 70% identical to at least 20 contiguous nucleotides of the coding sequence of the CYP51B gene;
- iii) a sense sequence being at least 70% identical to at least 20 contiguous nucleotides of the coding sequence of the CYP51C gene;
- and
- iv) antisense sequences being substantially complementary to the sense sequences (i), (ii), and (iii);
- wherein the arrangement of the sequences within the dsRNA molecule allows hybridization of each sense sequence with its substantially complementary antisense sequence.
(canceled)
A dsRNA molecule capable of inhibiting the expression of at least one CYP51 gene from a fungal or oomycete phytopathogen selected from Table 1, the dsRNA comprising:
- a sense sequence being at least 70% identical to at least 20 contiguous nucleotides of the coding sequence of said at least one CYP51 gene, and
- an antisense sequence being substantially complementary to said sense sequence,
- wherein the arrangement of the sequences within the dsRNA molecule allows hybridization of said sense sequence and said corresponding antisense sequence.
Dependencies are inferred from the claim text.