Pcr device and pcr method
AssigneeFluid Incorporated
InventorShuzo Hirahara
Life of this patent
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Abstract
A reaction container 10 of the PCR device 1 has a tubular ring channel 20 formed by a substrate 11, a channel forming plate 12, and a cover plate 13. The ring channel 20 comprises an electrode pair 21 disposed, in a diametrical direction of an inner surface of one side wall of the ring channel 20, to face each other with a horizontal gap interposed therebetween, and is vertically erected. The gap length of the electrode pair 21 is across the entire width within a cross-section of the ring channel 20.
Claims4
A PCR device comprising:
- an annular container, a flow path cross-section of which is rectangular, which is in a circular annular shape as a whole and disposed by vertical installation, to carry out polymerase chain reaction (PCR);
- an electrode pair consisting of two electrodes disposed on one inner surface of opposed substrates to form the annular container, disposed to face each other with a gap interposed therebetween, said gap extending across an entire width of a flow of a reaction solution, intersecting the flow, and having a horizontal gap center line;
- and
- a control unit which applies an AC voltage to the electrode pair to make an AC current flow through the reaction solution, thereby generating Joule heat to make the reaction solution flow upward and control the reaction solution in temperature.
A PCR device comprising:
- an annular container, a flow path cross-section of which is rectangular, which is in a circular annular shape as a whole and disposed by vertical installation, to carry out polymerase chain reaction (PCR);
- an electrode pair consisting of electrodes each of which is disposed on respective inner surfaces of opposed substrates to form the annular container, disposed to face each other with a gap interposed therebetween, said gap extending across an entire width of a flow of a reaction solution, intersecting the flow, and having a horizontal gap center line;
- and
- a control unit which applies an AC voltage to the electrode pair to make an AC current flow through the reaction solution, thereby generating Joule heat to make the reaction solution flow upward and control the reaction solution in temperature.
A PCR method of providing an electrode pair consisting of two electrodes disposed on one inner surface of opposed substrates to form an annular container, a flow path cross-section of which is rectangular, and which is in a circular annular shape as a whole and disposed by vertical installation, to carry out polymerase chain reaction (PCR), and disposed to face each other with a gap interposed therebetween, said gap extending across an entire width of a flow of a reaction solution, intersecting the flow, and having a horizontal gap center line, and applying an AC voltage to the electrode pair to make an AC current flow through the reaction solution, thereby generating Joule heat to make the reaction solution flow upward and control the reaction solution in temperature.
A PCR method of providing an electrode pair consisting of electrodes each of which is disposed on respective inner surfaces of opposed substrates to form the annular container, a flow path cross-section of which is rectangular, and which is in a circular annular shape as a whole and disposed by vertical installation, to carry out polymerase chain reaction (PCR), and disposed to face each other with a gap interposed therebetween, said gap extending across an entire width of a flow of a reaction solution, intersecting the flow, and having a horizontal gap center line, and applying an AC voltage to the electrode pair to make an AC current flow through the reaction solution, thereby generating Joule heat to make the reaction solution flow upward and control the reaction solution in temperature.
Dependencies are inferred from the claim text.