Bacteria engineered for ester production
AssigneeThe Regents of the University of California
InventorsShota ATSUMI, Gabriel RODRIGUEZ, Yohei TASHIRO
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Abstract
The present disclosure provides recombinant bacteria with elevated 2-keto acid decarboxylase and alcohol transferase activities. Some recombinant bacteria further have elevated aldehyde dehydrogenase activity. Some recombinant bacteria further have reduced alcohol dehydrogenase and/or aldehyde reductase activity. Methods for the production of the recombinant bacteria, as well as for use thereof for production of various esters are also provided.
Claims38
A bacterium comprising a recombinant polynucleotide encoding an alcohol transferase (ATF) and either a 2-keto acid decarboxylase (KDC) or a 2-ketoisovalerate dehydrogenase enzyme complex (KIVDH), wherein expression of the ATF and either the KDC or the KIVDH results in an increase in production of an ester as compared to a corresponding bacterium lacking the recombinant polynucleotide.
A bacterium comprising a recombinant polynucleotide encoding an alcohol transferase (ATF) and a LuxCDE enzyme system, wherein expression of the ATF and the LuxCDE enzyme system results in an increase in production of an ester as compared to a corresponding bacterium lacking the recombinant polynucleotide.
A bacterium comprising a recombinant polynucleotide encoding an acetyl transferase (AcTF) and either a 2-keto acid decarboxylase (KDC) or a 2-ketoisovalerate dehydrogenase enzyme complex (KIVDH), wherein expression of the AcTF and either the KDC or the KIVDH results in an increase in production of an ester as compared to a corresponding bacterium lacking the recombinant polynucleotide.
A bacterium comprising a recombinant polynucleotide encoding:
- (a) an alcohol transferase (ATF) or acetyl transferase (AcTF);
- (b) a 2-keto acid decarboxylase (KDC) or a 2-ketoisovalerate dehydrogenase enzyme complex (KIVDH);
- and
- (c) an acetolactate synthase (ALS), an acetohydroxy acid isomeroreductase (AHIR), and a dihydroxy acid dehydratase (DHAD),
- wherein expression of the ATF or the AcTF, the KDC or the KIVDH, and the ALS, AHIR and DHAD results in an increase in production of an ester as compared to a corresponding bacterium lacking the recombinant polynucleotide.
A bacterium comprising a recombinant polynucleotide encoding acetate kinase A (AckA) and phosphate acetyltransferase (Pta), wherein expression of AckA and Pta permits the bacterium to grow in culture medium comprising acetate.
A method for producing isobutyl isobutyrate, the method comprising:
- (a) providing a bacterium encoding an alcohol transferase (ATF) and a 2-ketoisovalerate dehydrogenase enzyme complex (KIVDH);
- and
- (b) culturing the bacterium of (a) in culture medium comprising isobutanol and under conditions suitable for the conversion of the isobutanol to isobutyl isobutyrate,
- wherein expression of the ATF and the KIVDH results in an increase in production of isobutyl isobutyrate as compared to a corresponding bacterium lacking the recombinant polynucleotide, when cultured under the same conditions.
Dependencies are inferred from the claim text.