Designing a soluble full-length hiv-1 gp41 trimer
AssigneeThe Catholic University of America
InventorsVenigalla B. RAO, Guofen GAO
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Abstract
Described herein is a soluble HIV-1 retrovirus transmembrane glycoprotein gp41 trimer (Soc-gp41M-Fd) containing a partial ectodomain and the cytoplasmic domain, that is fused to the small outer capsid (Soc) protein of bacteriophage T4 and the Foldon domain of the bacteriophage T4 fibritin (Fd). The gp41 trimer that has a prehairpin structure could be utilized to understand the mechanism of viral entry and as a candidate for development of HIV-1 vaccines, diagnostics and therapeutics. Other secondary embodiments of the gp41 proteins containing different modifications are also disclosed. According to one embodiment, the gp41 trimer is further attached to a cell penetration peptide (CPP). Methods of producing gp41 trimers are also disclosed.
Claims5
1-12. (canceled)
A method comprising the following steps:
- (a) expressing a fusion protein from an expression vector containing a DNA fragment encoding a fusion protein comprising amino acid sequence SEQ ID NO:
- 6,
- (b) purifying the fusion protein from step (a) to thereby form a fusion protein in purified form comprising amino acid sequence SEQ ID NO:
- 6.
16-34. (canceled)
Dependencies are inferred from the claim text.