Method for Producing Myo-Inositol and Myo-Inositol Derivative
AssigneeAsahi Kasei Chemicals Corporation
InventorsKazunobu Konishi, Shinichi Imazu, Mayumi Sato
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Abstract
To impart significantly improved myo-inositol producing capability, suitable for use in recombinant DNA techniques and synthetic biology methods, to a host microorganism that does not possess an endogenous myo-inositol biosynthesis pathway, such as Escherichia coli. Inositol monophosphatase activity is strengthened in a transformant obtained by introducing a myo-inositol biosynthesis pathway into a host microorganism that does not possess an endogenous myo-inositol biosynthesis pathway.
Claims23
A method of producing myo-inositol or a myo-inositol derivative, comprising:
- (1) preparing a transformant of a microorganism that does not express endogenous inositol-1-phosphate synthase, the transformant containing at least an inositol-1-phosphate synthase-encoding exogenous gene introduced expressibly into the transformant, and the transformant having a gene recombination or mutation to induce overproduction of functional inositol monophosphatase or activation of inositol monophosphatase within the transformant;
- and
- (2) contacting the transformant with a carbon source that is converted to the compound of claim 21 by the transformant under conditions suited to growth and/or maintenance of the transformant.
(canceled)
A transformant of a microorganism that does not express endogenous inositol-1-phosphate synthase, the transformant containing at least an inositol-1-phosphate synthase-encoding exogenous gene introduced expressibly into the transformant, and the transformant having a gene recombination or mutation to induce overproduction of functional inositol monophosphatase or activation of inositol monophosphatase within the transformant.
A compound represented by the following structural formula:
- (Chemical Formula 2)
Dependencies are inferred from the claim text.