Glycine max resistance gene(s) and use thereof to engineer plants with broad-spectrum resistance to fungal pathogens and pests

A method for conferring or improving Fusarium resistance in a plant, said method comprising:

• transforming said plant with a nucleic acid molecule comprising a heterologous sequence operably linked to a promoter that induces transcription of said heterologous sequence in a plant cell;
• and regenerating stably transformed plants, wherein said heterologous sequence comprises a nucleic acid molecule that encodes one or more Fusarium resistance protein sequences selected from the group consisting of:
• Glycine max salicylic acid methyl transferase, Glycine max ankyrin repeat-containing protein, Glycine max sieve element-occlusion protein, and F. virguliforme polyamine oxidase.

The method of 11, wherein said plant is a dicot.

The method of 11, wherein said dicot is soybean.

A method for conferring or improving Fusarium resistance of a plant, said method comprising:

• stably introducing into the genome of a plant, at least one nucleotide construct comprising a Fusarium resistance nucleic acid molecule operably linked to a heterologous promoter that drives expression in a plant cell, wherein said nucleic acid molecule encodes a polypeptide selected from the group consisting of:
• Glycine max salicylic acid methyl transferase, Glycine max ankyrin repeat-containing protein, Glycine max sieve element-occlusion protein, Glycine max disease susceptibility 1 (GmDS1), Glycine max-occlusion protein, and F. virguliforme polyamine oxidase.

A method of screening a plant for Fusarium resistance comprising:

• assaying said plant for the presence of a Fusarium resistance nucleic acid gene or fragment thereof, said nucleic acid encoding a protein selected from the group consisting of Glycine max salicylic acid methyl transferase, Glycine max ankyrin repeat-containing protein, Glycine max sieve element-occlusion protein, and F. virguliforme polyamine oxidase.

A modified plant with improved broad-spectrum pathogen resistance, compared to a corresponding plant with no such modification;

• said modified plant having a heterologous nucleotide sequence which includes a Fusarium resistance nucleic acid sequence, said Fusarium resistance nucleic acid sequence encoding a protein selected from the group consisting of:
• Glycine max salicylic acid methyl transferase, Glycine max ankyrin repeat-containing protein, Glycine max sieve element-occlusion protein, Glycine max disease susceptibility 1 (GmDS1), Glycine max-occlusion protein, and F. virguliforme polyamine oxidase.

A method for conferring or improving pathogen resistance in a plant, said method comprising:

• transforming said plant with a nucleic acid molecule comprising a heterologous sequence operably linked to a promoter that induces transcription of said heterologous sequence in a plant cell;
• and regenerating stably transformed plants, wherein said heterologous sequence comprises a nucleic acid molecule that encodes one or more Fusarium resistance protein sequences selected from the group consisting of:
• Glycine max salicylic acid methyl transferase, Glycine max ankyrin repeat-containing protein, Glycine max sieve element-occlusion protein, Glycine max disease susceptibility 1 (GmDS1), Glycine max-occlusion protein, and F. virguliforme polyamine oxidase.

The method of 29, wherein said plant is a dicot.

The method of 29, wherein said dicot is soybean.

A method for conferring or improving pathogen resistance of a plant, said method comprising:

• stably introducing into the genome of a plant, at least one nucleotide construct comprising a Fusarium resistance nucleic acid molecule operably linked to a heterologous promoter that drives expression in a plant cell, wherein said nucleic acid molecule encodes a polypeptide selected from the group consisting of:
• Glycine max salicylic acid methyl transferase, Glycine max ankyrin repeat-containing protein, Glycine max sieve element-occlusion protein, Glycine max disease susceptibility 1 (GmDS1), Glycine max-occlusion protein, and F. virguliforme polyamine oxidase.

A method of screening a plant for pathogen resistance comprising:

• assaying said plant for the presence of a Fusarium resistance nucleic acid gene or fragment thereof, said nucleic acid encoding a protein selected from the group consisting of Glycine max salicylic acid methyl transferase, Glycine max ankyrin repeat-containing protein, Glycine max sieve element-occlusion protein, and F. virguliforme polyamine oxidase.

A method of plant breeding for Fusarium resistance comprising:

• identifying a plant with a heterologous Fusarium resistance nucleic acid encoding an Glycine max salicylic acid methyl transferase, Glycine max ankyrin repeat-containing protein, Glycine max sieve element-occlusion protein, Glycine max disease susceptibility 1 (GmDS1), Glycine max-occlusion protein, and F. virguliforme polyamine oxidase or the protein product thereof, selecting said resistant plant for use a parent plant;
• crossing said parent plant with itself or a second plant, so that the Fusarium resistance trait is passed to progeny seed;
• and harvesting progeny seed from said parent plant.