Plants comprising events pp009-401, pp009-415, and pp009-469, compositions, sequences, and methods for detection thereof
AssigneeOMS Investments, Inc.
InventorsROBERT HARRIMAN, LISA LEE, DAVID STALKER, Rebecca TORISKY
Life of this patent
Estimating the term…
Abstract
The invention provides glyphosate tolerant transgenic turfgrass plants, plant material, and seeds that have a specific transformation event. Also provided are assays for detecting the presence of the event. The invention also provides sequences for a variant EPSPS gene and a GAO2X gene, cassettes, and plants comprising the variant EPSPS gene and a GAO2X gene.
Claims113
Seed of Kentucky bluegrass comprising event Pp009-401, event Pp009-415, or event Pp009-469, a representative sample of seed comprising event Pp009-401, event Pp009-415, and event Pp009-469 having been deposited under ATCC Accession Nos. PTA-120354, PTA-120353, and PTA-120355, respectively.
A method for producing Kentucky bluegrass plant or seed comprising crossing a Kentucky bluegrass plant comprising event Pp009-401, event Pp009-415, or event Pp009-469 with a plant lacking event Pp009-401, event Pp009-415, or event Pp009-469, and planting seed obtained from said cross, wherein said seed comprises event Pp009-401, event Pp009-415, or event Pp009-469.
Kentucky bluegrass genomic DNA comprising event Pp009-401, event Pp009-415, or event Pp009-469.
A method for producing Kentucky bluegrass plant or seed comprising selfing or crossing a Kentucky bluegrass plant comprising event Pp009-401, event Pp009-415, or event Pp009-469 with a plant lacking event Pp009-401, event Pp009-415, or event Pp009-469, and planting seed obtained from said cross.
An isolated nucleic acid comprising the nucleotide sequence of SEQ ID NO:
- 10, SEQ ID NO:
- 11, SEQ ID NO:
- 12, or SEQ ID NO:
- 13.
An isolated nucleic acid comprising the nucleotide sequence of SEQ ID NO:
- 10.
An isolated nucleic acid comprising the nucleotide sequence of SEQ ID NO:
- 11.
An isolated nucleic acid comprising the nucleotide sequence of SEQ ID NO:
- 12.
An isolated nucleic acid comprising the nucleotide sequence of SEQ ID NO:
- 13.
An isolated cassette comprising the nucleotide sequence of SEQ ID NO:
- 10 or SEQ ID NO:
- 11.
An isolated cassette comprising the nucleotide sequence of SEQ ID NO:
- 10.
An isolated cassette comprising the nucleotide sequence of SEQ ID NO:
- 11.
An isolated plasmid comprising the nucleotide sequence of SEQ ID NO:
- 10, SEQ ID NO:
- 11, SEQ ID NO:
- 12, or SEQ ID NO:
- 13.
An isolated plasmid comprising the nucleotide sequence of SEQ ID NO:
- 10.
An isolated plasmid comprising the nucleotide sequence of SEQ ID NO:
- 11.
An isolated plasmid comprising the nucleotide sequence of SEQ ID NO:
- 12.
An isolated plasmid comprising the nucleotide sequence of SEQ ID NO:
- 13.
An isolated cell comprising the nucleotide sequence of SEQ ID NO:
- 10, SEQ ID NO:
- 11, SEQ ID NO:
- 12, or SEQ ID NO:
- 13.
An isolated cell comprising the nucleotide sequence of SEQ ID NO:
- 10.
An isolated cell comprising the nucleotide sequence of SEQ ID NO:
- 11.
An isolated cell comprising the nucleotide sequence of SEQ ID NO:
- 12.
An isolated cell comprising the nucleotide sequence of SEQ ID NO:
- 13.
A Kentucky bluegrass plant, cell, plant part, or seed comprising the nucleotide sequence of SEQ ID NO:
- 10, SEQ ID NO:
- 11, SEQ ID NO:
- 12, or SEQ ID NO:
- 13.
A seed of Kentucky bluegrass comprising the nucleotide sequence of SEQ ID NO:
- 10, SEQ ID NO:
- 11, SEQ ID NO:
- 12, or SEQ ID NO:
- 13.
A method for producing a Kentucky bluegrass plant that tolerates application of glyphosate comprising sexually crossing a first parental Kentucky bluegrass comprising the nucleic acid of SEQ ID NO:
- 10 or 12 and a second parental plant that lacks the nucleic acid of SEQ ID NO:
- 10 or 12 or that lacks glyphosate tolerance, thereby producing a plurality of progeny plants.
A method for producing a Kentucky bluegrass plant that tolerates application of glyphosate comprising:
- (a) sexually crossing a first parental Kentucky bluegrass the nucleic acid of SEQ ID NO:
- 10 or 12 and a second parental plant that lacks the nucleic acid of SEQ ID NO:
- 10 or 12 or that lacks glyphosate tolerance, thereby producing a plurality of progeny plants;
- and (b) selecting a progeny plant that tolerates application of glyphosate.
A turfgrass stand, lawn, sports field, or golf course comprising a Kentucky bluegrass plant comprising the nucleic acid of SEQ ID NO:
- 10 or 12.
A method of controlling weeds in a turfgrass stand of Kentucky bluegrass the nucleic acid of SEQ ID NO:
- 10 or 12 comprising the step of applying a glyphosate containing herbicide formulation to the turfgrass stand.
A plant cell comprising the nucleotide sequence of SEQ ID NO:
- 10, SEQ ID NO:
- 11, SEQ ID NO:
- 12, or SEQ ID NO:
- 13.
A plant cell comprising the event Pp009-401, event Pp009-415, or event Pp009-469.
A plant comprising the event Pp009-401, event Pp009-415, or event Pp009-469.
A plant comprising the nucleotide sequence of SEQ ID NO:
- 10, SEQ ID NO:
- 11, SEQ ID NO:
- 12, or SEQ ID NO:
- 13.
A transgenic plant comprising the nucleotide sequence of SEQ ID NO:
- 10.
A transgenic plant comprising the nucleotide sequence of SEQ ID NO:
- 11.
A transgenic plant comprising the nucleotide sequence of SEQ ID NO:
- 12.
A transgenic plant comprising the nucleotide sequence of SEQ ID NO:
- 13.
An isolated DNA molecule with at least 80% homology to the nucleic acid sequence of SEQ ID NO:
- 1, SEQ ID NO:
- 2, SEQ ID NO:
- 3, SEQ ID NO:
- 4, SEQ ID NO:
- 5, SEQ ID NO:
- 6, SEQ ID NO:
- 7, SEQ ID NO:
- 8, SEQ ID NO:
- 9, SEQ ID NO:
- 10, SEQ ID NO:
- 11, SEQ ID NO:
- 12, or SEQ ID NO:
- 13.
A method of producing a polypeptide comprising expressing an expression vector in a host cell and harvesting the polypeptide.
Dependencies are inferred from the claim text.