Alkenol dehydratase variants
AssigneesGlobal Bioenergies, Scientist of Fortune, S.A.
InventorsPhilippe Marliere, Marc Delcourt, Sabine Mazaleyrat
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Abstract
Described are alkenol dehydratase variants having improved activity in catalyzing the conversion of a compound corresponding to the general formula CnH2nO into CnH2n-2+H2O with 3<n<7. In particular, described are alkenol dehydratase variants having, e.g., an improved activity in converting but-2-en-1-ol (crotyl alcohol) into 1,3 butadiene and/or an improved activity in converting but-3-en-2-ol into 1,3 butadiene and/or an improved activity in converting 3-methylbut-2-en-1-ol (prenol) or 3-methylbut-3-en-2-ol (isoprenol) into isoprene and/or an improved activity in converting 2,3-dimethyl-but-2-en-1-ol into dimethyl-butadiene.
Claims26
1-22. (canceled)
An alkenol dehydratase variant derived from an amino acid sequence at least 60% identical to SEQ ID NO:1, wherein said variant is capable of converting at least one alkenol compound corresponding to the general formula CnH2nO into a conjugated diene CnH2n-2 with an improved activity over the activity of the amino acid sequence from which the variant is derived, and wherein 3<n<7.
Dependencies are inferred from the claim text.