Methods for the prevention of aggregation of viral components

A method for producing a composition comprising Enteroviral particles, the method comprising:

• (a) purifying Enteroviral particles from a medium containing Enteroviral particles, whereby during at least a part of the purification a basic amino acid or a derivative thereof is present at a concentration sufficient to prevent or reduce aggregation of the Enteroviral particles;
• and, optionally, at least one of:
• (b) inactivating the Enteroviral particles;
• and,
• (c) formulating the Enteroviral particles,
• wherein the basic amino acid or derivative thereof is selected from the group consisting of arginine, lysine, histidine, arginine-HCl, lysine-HCl, histidine-HCl, agmatine, L-arginine ethyl ester dihydrochloride, tranexamic acid, DL-5-hydroxylysine hydrochloride, L-lysine methyl ester dihydrochloride, 3-methyl-L-histidine, salts thereof and combinations thereof.

A composition comprising viral particles of a virus that belongs to Paramyxoviridae or Orthomyxoviridae, and a basic amino acid or derivative thereof selected from the group consisting of:

• arginine, lysine, histidine, arginine-HCl, lysine-HCl, histidine-HCl, agmatine, L-arginine ethyl ester dihydrochloride, tranexamic acid, DL-5-hydroxylysine hydrochloride, L-lysine methyl ester dihydrochloride, 3-methyl-L-histidine, salts thereof and combinations thereof, wherein:
• (a) the total concentration of the basic amino acids or derivatives in the composition is higher than the total concentration of the basic amino acid or derivatives thereof as present in a culture medium;
• (b) the composition does not comprise a buffer;
• and/or,
• (c) the composition essentially consist of the viral particles, the basic amino acid or a derivative thereof in a total concentration of at least 0.01 mM and, optionally water.