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AssigneeUniversity of Warwick
InventorsPaul Thornalley, Naila Rabbani
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The disclosure relates to a fluorescence based assay for the detection of alpha, beta-dicarbonyl containing compounds, for example alpha-oxoaldehyde compounds such as glyoxal, methylglyoxal, hydroxypyruvaldehyde, erythrosone, 3-deoxy-erythrosone, ribosone, 3-deoxyribosone, glucosone, 3-deoxyglucosone and stereoisomers thereof and butan-2,3-dione that uses a fluorogenic probe which is stable under ambient conditions. The fluorogenic probes comprise a 1,2-diaminophenyl moiety. The diaminophenyl probes yield a 20-fold increase in quantum yield when they are a derivative of fluorescein (DAF), rhodamine (DAR), BODIPY or cyanine. The assay is preferably carried out at pH 4-8. Corresponding screening methods for modulators of alpha, beta-dicarbonyl compound concentrations are also disclosed.
A method for the detection of α,β-dicarbonyl compounds in a sample comprising the steps:
The method according to any one of the preceding claims wherein said α,β-dicarbonyl compound is a α-oxoaldehyde compound.
The method according to any one of the preceding claims wherein said sample is a biological sample.
The method according to 8 wherein said bodily fluid is selected from the group consisting of:
The method according to any one of the preceding claims wherein (1) said method is a method of diagnosis of a subject which has or is suspected of having a disease or condition associated with elevated levels of a α,β-dicarbonyl compound;
The method according to any one of the preceding claims wherein said fluorescent product is detected by excitation at between 435-445 nm and emission between 500-550 nm for 4,5-diaminofluorescein and similar appropriate wavelengths for 4,5-diaminorhodamine, 8-(3,4-diaminophenyl)-2,6-bis(2-carboxyethyl)-4,4-difluoro-1,3,5,7-tetramethyl-4-bora-3a,4a-diaza-s-indacene, o-diaminocyanine or a functional derivative thereof.
Dependencies are inferred from the claim text.