Method for production of reprogrammed cell using chromosomally unintegrated virus vector
AssigneeID Pharma Co., Ltd.
InventorsNoemi FUSAKI, Hiroshi BAN, Mamoru HASEGAWA, Yoshikazu YONEMITSU
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Abstract
An objective of the present invention is to provide vectors for conveniently and efficiently producing ES-like cells in which foreign genes are not integrated into the chromosome. The present inventors discovered methods for producing ES-like cells from somatic cells using chromosomally non-integrating viral vectors. Since no foreign gene is integrated into the chromosome of the produced ES-like cells, they are advantageous in tests and research, and immunological rejection and ethical problems can be avoided in disease treatments.
Claims10
1-39. (canceled)
A Sendai virus vector having a deletion of the F gene and comprising one or more mutations selected from the group consisting of G69E, T116A and A183S in the gene for the M protein, A262T, G264R and K461 G in the gene for the HN protein, L511F in the gene for the P protein, and N1197S and K1795E in the gene for the L protein, wherein said Sendai virus vector furthermore comprises one or more mutations selected from the group consisting of D433A, R434A and K437A in the gene for the P protein and Y942H, L1361C and L1558I in the gene for the L protein.
Dependencies are inferred from the claim text.