Process for production of insulin and insulin analogues
AssigneeBiogenomics Limited
InventorsArchana KRISHNAN, Sanjay SONAR, Damodar THAPPA
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Abstract
A process for production of insulin or insulin analogues by expression of Insulin or Insulin analogues through an expression vector in a host cell is provided. The expression vector includes a leader peptide of SEQ ID NO 3; a nucleotide sequence encoding an affinity tag linked to C-terminal end or N terminal end of nucleotide sequence of the leader peptide; and a nucleotide sequence encoding for a cleavage site ligated to nucleotide sequence of the leader peptide through nucleotide sequence encoding the affinity tag.
Claims15
A process for production of insulin or insulin analogues by expression of Insulin or Insulin analogues through an expression vector in a host cell, wherein said expression vector comprises:
- a leader peptide of SEQ ID NO 3;
- a nucleotide sequence encoding an affinity tag linked to C-terminal end or N terminal end of nucleotide sequence of said leader peptide;
- and
- a nucleotide sequence encoding for a cleavage site ligated to nucleotide sequence of said leader peptide through nucleotide sequence encoding said affinity tag.
A process for production of insulin or insulin analogues by expression of insulin or insulin analogues through an expression vector in a host cell, wherein said expression vector comprises:
- a leader peptide of SEQ ID NO 3;
- a nucleotide sequence encoding an affinity tag linked to C-terminal end or N-terminal end of nucleotide sequence of said leader peptide;
- a nucleotide sequence encoding for a cleavage site or Restriction Enzyme (RE) site ligated to nucleotide sequence of said leader peptide through nucleotide sequence encoding said affinity tag;
- a nucleotide sequence encoding a multiple cloning site (MCS) in upstream region of said leader peptide;
- a nucleotide sequence encoding ribosome binding site (RBS) ligated to N-terminus or C-terminus of said leader peptide;
- a nucleotide sequence encoding a promoter or operator in the downstream of said ribosome binding site;
- and
- a nucleotide sequence encoding an antibiotic selection marker in upstream region of said promoter/operator sequence.
Dependencies are inferred from the claim text.