Spatial Positioning of Spectrally Labeled Beads
AssigneeLife Technologies Corporation
InventorsStephen Empedocles, Andrew WATSON, Jian Jin
Life of this patent
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Abstract
Devices, systems, kits, and methods for detecting and/or identifying a plurality of spectrally labeled bodies well-suited for performing multiplexed assays. By spectrally labeling the beads with materials which generate identifiable spectra, a plurality of beads may be identified within the fluid. Reading of the beads is facilitated by restraining the beads in arrays, and/or using a focused laser.
Claims16
A spectral label identification method comprising:
- spatially restraining a first spectrally labeled body at a site in an array of sites;
- spatially restrained a plurality of labeled bodies behind the first body so as to define an array;
- dispersing a plurality of spectra from the spatially restrained bodies simultaneously across the sensor surface;
- and
- identifying the array of bodies from the spectrum, wherein the plurality of spectrally labeled bodies are simultaneously spatially restrained as an array when the first body is restrained.
A spectral label identification method comprising:
- spatially restraining a first spectrally labeled body at a site in an array of sites;
- spatially restraining a plurality of labeled bodies behind the first body so as to define an array.
- sensing a first and second spectra with a scanning system by moving a sensing field between the bodies of the array;
- and
- identifying the array of bodies from the spectrum, wherein the plurality of spectrally labeled bodies are simultaneously spatially restrained as an array when the first body is restrained.
A spectral label identification method comprising:
- spatially restraining a first spectrally labeled body at a site in an array of sites in an array of sites;
- spatially restraining an array of bodies in the array by an array of discrete binging sites, the binding sites comprising material capable of binding bodies;
- sensing a first and second spectra with a scanning system by moving a sensing field between the bodies of the array;
- and
- identifying the array of bodies from the spectrum, wherein the plurality of spectrally labeled bodies are simultaneously spatially restrained as an array when the first body is restrained.
A spectral label identification method comprising:
- spatially restraining a first spectrally labeled body at a site in an array of sites in an array of sites;
- spatially restraining an array of bodies in the array by an array of discrete bringing sites, the binding sites comprising material capable of binding bodies;
- dispersing a plurality of spectra from the spatially restrained bodies simultaneously across the sensor surface;
- and
- identifying the array of bodies from the spectrum, wherein the plurality of spectrally labeled bodies are simultaneously spatially restrained as an array when the first body is restrained.
A multiplex assay system comprising:
- A plurality of bodies released in a fluid, the bodies having labels for generating identifiable spectra;
- An energy transmitter coupled to the fluid so as to spatially restrain an array of bodies with a restraining energy beam;
- and
- A sensor oriented to receive the spectrum which is simultaneously dispersed from the plurality of spatially restrained bodies across the sensor surface.
Dependencies are inferred from the claim text.