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US 2016/0160249 A1Published application
Increasing methionine yield
AssigneeMetabolic Explorer
InventorsRainer FIGGE, Philippe SOUCAILLE, Guillaume BARBIER, Gwénaëlle BESTEL-CORRE, Cédric BOISART, Michel CHATEAU
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Abstract
Process for the production of methionine or its derivatives by culturing a microorganism in an appropriate culture medium comprising a source of carbon and a source of sulfur. The microorganism and/or the culture medium are modified in such way that the methionine/carbon source yield is increased. The isolation of methionine or its derivates from the fermentation medium is also described.
Claims5
A method for the production of methionine, a derivative thereof, or a precursor in a fermentative process comprising the following steps:
- culturing a modified microorganism in an appropriate culture medium comprising a source of carbon and a source of sulfur, and
- recovering methionine from the culture medium,
- wherein said modified microorganism is genetically modified to increase the yield of methionine per amount of carbone source,
- wherein the growth and biomass production of the said microorganism is limited by limiting or starving said microorganism for phosphate in the culture medium, and
- wherein said microorganism exhibits an improved methionine/carbon source yield of at least 12% gram/gram as compared to the microorganism without the specified modifications and/or the culture medium without the modifications
- wherein for said methionine/carbon source yield, the carbon source is hexoses, pentoses, monosaccharides, disaccharides, oligosaccharides, molasses, starch or its derivatives, hemicelluloses, glycerol, and combinations thereof.
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Dependencies are inferred from the claim text.