Vaccines for diseases caused by viruses of the family of reoviridae
AssigneeStichting Dienst Landbouwkundig Onderzoek
InventorsPetrus Antonius van Rijn, Hendricus Gerardus Petrus van Gennip, Sandra Gerarda Petra van de Water, Femke Feenstra
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Abstract
The invention relates to methods for producing a propagation-competent strain of a mutant Reoviridae virus, and to a propagation-competent strain of a mutant Reoviridae virus that is obtainable by a method of the invention. The invention further relates to a propagation-competent strain of a mutant Reoviridae virus, comprising a deletion of a genetic region that is relevant for propagation of the virus, and to a vaccine, comprising a propagation-competent strain of a mutant Reoviridae virus.
Claims15
A method for producing a propagation-competent strain of a mutant Reoviridae virus comprising a functional deletion in a viroporin-coding region that abolishes expression of all endogenous viroporin proteins, the method comprising:
- a) introducing the mutant Reoviridae virus in a non-complementing cell line;
- b) passaging said mutant virus in said cell line until virus is produced;
- and
- c) propagating the propagation-competent strain of the mutant virus.
A propagation-competent strain of a mutant Reoviridae virus, comprising a functional deletion in a viroporin-coding region.
Dependencies are inferred from the claim text.