Method and device for bioassays
AssigneeAbionic SA
InventorsNicolas DURAND, Iwan MÄRKI, Annick MAYOR, Stéphane BROILLET
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Abstract
A method and device for multiplexing and calibrating rapid quantification of biomolecules present in a nanofluidic biosensor composed by a nanoslit (210) is claimed. In particular, the present invention relates to a novel concept defining multiple different functionalized areas containing biomarkers. Functionalized areas can also being structured to decrease the biomarkers density in the nanoslit. The present concept enables the multiplexed quantification biomolecular interactions of interest in the same nanofluidic biosensor.
Claims13
A biosensor for detecting and quantifying fluorescently-labeled biomolecules, said biosensor comprising:
- a lower substrate;
- an upper substrate stacked on said lower substrate;
- a nanoslit formed between said lower substrate and said upper substrate, said nanoslit comprising
- a plurality of functionalized areas;
- and
- a plurality of biomarkers immobilized on said plurality of functionalized areas,
- wherein each one of said plurality of functionalized areas comprises at least one of said plurality of biomarkers,
- a lateral input aperture in direct contact with said nanochannel, for letting a solution containing biomolecules enter said nanochannel;
- and
- a lateral output aperture in direct contact with said nanochannel, for driving said solution through said nanoslit.
Dependencies are inferred from the claim text.