Polyarginine-coated magnetic nanovector and methods of use thereof
AssigneeUniversity of Washington through its Center for Commercialization
InventorsMiqin Zhang, Omid Veiseh, Chen Fang, Forrest Kievit
Life of this patent
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Abstract
Polyarginine-coated nanoparticle, and methods for making and using the nanoparticle. The nanoparticle can have a core that includes a material that imparts magnetic resonance imaging activity to the particle and, optionally, include one or more of an associated therapeutic agent, targeting agent, and diagnostic agent.
Claims18
A method for introducing a nanovector into a cell via transcytosis, comprising contacting a cell with a nanoparticle comprising:
- (a) a core having a surface and comprising a core material;
- and
- (b) a polyarginine coating covalently coupled to the surface of the core.
A method for silencing or reducing the expression level of a gene, comprising contacting a cell of interest with a nanoparticle comprising a vector effective to silence or reduce the expression level of the gene, wherein the nanoparticle comprises:
- (a) a core having a surface and comprising a core material;
- and
- (b) a polyarginine coating covalently coupled to the surface of the core.
A method for detecting cells or tissues by magnetic resonance imaging, comprising:
- (a) contacting cells or tissues of interest with a nanoparticle, wherein the nanoparticle comprises:
- (i) a core having a surface and comprising a core material;
- and
- (ii) a polyarginine coating covalently coupled to the surface of the core;
- and
- (b) measuring the level of binding of the nanoparticle, wherein an elevated level of binding, relative to normal cells or tissues, is indicative of binding to the cells or tissues of interest.
Dependencies are inferred from the claim text.