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US 2016/0145599 A1Published application
Mutant Yeasts Having an Increased Production of Lipids and of Citric Acid
AssigneesInstitut National de la Recherche Agronomique, Centre National De La Recherche Scientifique
InventorsJean-Marc NICAUD, Athanasios BEOPOULOS, Seraphim PAPANIKO-LAOU, Thierry DULERMO
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Abstract
The present invention relates to a mutant yeast strain, in which at least the expression or the activity of the 2-methyl-citrate dehydratase is inhibited, and to the use of said strain for the production of lipids and of citric acid.
Claims15
A mutant yeast strain, wherein:
- (a) the expression or the activity of the endogenous 2-methylcitrate dehydratase (EC 4.2.1.79) of said strain is inhibited,
- (b) the expression or the activity of at least one of the following proteins of said strain is inhibited:
- (i) the endogenous acyl-coenzyme A oxidases (EC 6.2.1.3),
- (ii) the endogenous multifunctional beta-oxidation protein (EC 4.2.1.74),
- (iii) the endogenous 3-oxoacyl-coenzyme A thiolase (EC 2.3.1.16),
- (iv) one or more of the endogenous proteins encoded by a PEX gene involved in yeast peroxisome metabolism,
- (v) one or more of the endogenous triacylglycerol lipases (EC 3.1.1.3), and
- (vi) the endogenous glycerol-3-phosphate dehydrogenases (EC 1.1.99.5), and
- (c) at least one of the following proteins or genes of said strain is overexpressed:
- (i) endogenous genes encoding a glycerol-3-phosphate dehydrogenase (NAD(+)) (EC 1.1.1.18),
- (ii) an acetyl-CoA carboxylase (EC 6.4.1.2),
- (iii) an acyl-CoA:diacylglycerol acyltransferase (EC 2.3.1.20),
- (iv) an ATP citrate lyase (EC 2.3.3.8),
- (v) a malic enzyme (EC 1.1.1.40),
- (vi) an acetyl-CoA synthetase (EC 6.2.1.1),
- (vii) a Delta(9)-desaturase (EC 1.14.19.1),
- (viii) a Delta(12)-desaturase (EC 1.14.19.6) and
- (ix) an invertase (EC 3.2.1.26).
15 more elements
A method for increasing the lipid and/or citric acid production of a yeast strain, said method comprising inhibiting the expression or the activity of 2-methylcitrate dehydratase in said yeast strain.
(canceled)
Dependencies are inferred from the claim text.