Shear Wave Imaging Method and Installation for Collecting Information on a Soft Solid

An in vitro method of prognosing outcome of an inflammatory bowel disease in a patient suffering from an inflammatory bowel disease comprising a step of determining the number and/or concentration and/or proportion of T regulatory lymphocytes with a CD4+ CD8ααlow Foxp3neg phenotype which are specific for F prausnitzii in a biological sample from the patient.

An in vitro method of predicting whether a subject is at risk of developing an inflammatory bowel disease, said method comprising:

• a) determining the number and/or concentration and/or proportion of T regulatory lymphocytes with a CD4+ CD8ααlow Foxp3neg phenotype which are specific for F prausnitzii in a biological sample from the subject;
• b) predicting from the result of step (a) if the subject is at risk of suffering from an inflammatory bowel disease, wherein a low value at step (a), relative to a reference value for a sample of the same nature, indicates that said subject is at risk of developing an inflammatory bowel disease.

An in vitro method of monitoring the efficacy of a preventive or curative treatment of an inflammatory bowel disease, the method comprises a step of monitoring the number and/or concentration and/or proportion of T regulatory lymphocytes with a CD4+ CD8ααlow Foxp3neg phenotype which are specific for F prausnitzii in a biological sample from the subject during the treatment.

8-10. (canceled)

A kit for diagnosing, prognosing and/or predicting the risk of developing an IBD, and/or for monitoring the efficacy of a treatment of an IBD, wherein said kit comprises means for determining the number and/or concentration and/or proportion of T regulatory lymphocytes with a CD4+ CD8ααlow Foxp3neg phenotype specific for F prausnitzii.

A method for treating or preventing an inflammatory bowel disease in a patient in need thereof, comprising a step of administering to said patient a therapeutically effective amount of isolated T regulatory lymphocytes with a CD4+ CD8ααlow Foxp3neg phenotype which are specific for F. prausnitzii.