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US 2016/0130602 A1Published application
Means and methods for yield performance in plants
AssigneeUniversiteit Gent
InventorsDirk Gustaaf Inzé, Hannes Claeys, Hilde Nelissen, Xiaohuan Sun
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Abstract
This disclosure relates to the field of plant molecular biology; more particularly to the field of agriculture; even more particularly to the field of improving the yield of plants. This disclosure provides chimeric genes and constructs that can be used to enhance the yield in plants and crops.
Claims18
A chimeric gene construct comprising the following operably linked DNA elements:
- a) the promoter region of a plant GA2 oxidase gene,
- b) a DNA region encoding a plant CYP78A5 protein or a functional orthologue thereof with an amino acid identity of at least 55%, and
- c) a 3′ end region comprising transcription termination and polyadenylation signals functioning in cells of a plant.
A plant, plant cell, or plant seed comprising an exogenous gene comprising the following operably linked DNA elements:
- a plant GA2 oxidase gene promoter region,
- a DNA region encoding a plant CYP78A5 protein or a functional orthologue thereof with an amino acid identity of at least 55% to plant CYP78A5 protein, and
- a 3′ end region comprising transcription termination and polyadenylation signals functioning in cells of a plant.
A method of producing a plant, the method comprising:
- introducing into or transforming the plant with a polynucleotide comprising the following operably linked DNA elements:
- a plant GA2 oxidase gene promoter region,
- a DNA region encoding a plant CYP78A5 protein or a functional orthologue thereof with an amino acid identity of at least 55% to plant CYP78A5 protein, and
- a 3′ end region comprising transcription termination and polyadenylation signals functioning in cells of the plant;
- and
- selecting a plant with a stable expression of the polynucleotide.
3 more elements
Dependencies are inferred from the claim text.