Sequencer pretreatment device and method thereof

A sequencer pretreatment device comprising:

• a suction and discharge mechanism configured to suck and discharge gas;
• a nozzle head having one or more nozzles configured to detachably mount a dispensing tip communicated with the suction and discharge mechanism and capable of sucking and discharging liquid;
• a container group comprising at least a reaction vessel and liquid accommodating portions for accommodating various liquids including magnetic particle suspension;
• a moving mechanism configured to cause relative movement among the nozzles and the container group;
• a magnetic unit configured to exert and remove from, from outside, a magnetic field to and from the dispensing tip mounted to the nozzle;
• and
• a control unit configured to control at least the suction and discharge mechanism, moving mechanism, and magnetic unit based on an external signal,
• wherein the control unit comprises:
• an extraction control unit configured to mix and stir a sample, extraction reagent solution, and magnetic particle suspension accommodated in the container group and to extract nucleic acid;
• a fragmentation producing control unit configured to fragmentate the extracted nucleic acid by mixing with fragmentation solution accommodated in the container group and to produce, from the fragmentated nucleic acid, a fragment of base sequence having the number of bases within a predetermined range corresponding to a sequencer using magnetic particle suspension;
• and
• an amplification pretreatment control unit configured to control such that a predetermined volume of solution containing the produced fragment is dispensed by the predetermined dispensing tip into the reaction vessel together with amplification solution for mixing therewith, and
• the fragmentation producing control unit is configured to control such that the fragment is produced by mixing at least the fragmentated nucleic acid, the magnetic particle suspension, and a binding promoter accommodated in the container group by the dispensing tip, allowing the fragment to bind with a magnetic particle, and producing a fragment of base sequence having the number of bases within a predetermined range corresponding to a sequencer or the other fragments by adding dissociation solution accommodated in the container group such that a desired concentration of the binding promoter is obtained based on a relationship, between a concentration of the binding promoter and the number of bases in the fragment of base sequence, that have been obtained in advance by electrophoresis and causing the fragment to be dissociated from the magnetic particle.