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US 2016/0115532 A1Published application
High sensitivity mutation detection using sequence tags
AssigneeSequenta, Inc.
InventorMalek Faham
Life of this patent
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AssigneeSequenta, Inc.
InventorMalek Faham
Estimating the term…
The invention is directed to methods for increasing the sensitivity of high throughput sequencing, particularly for distinguishing true rare mutations from amplification, sequencing and other sample processing errors that occur in sequencing techniques. In one aspect, methods of the invention includes steps of (a) preparing templates from nucleic acids in a sample; (b) labeling by sampling the templates to form tag-template conjugates, wherein substantially every template of a tag-template conjugate has a unique sequence tag; (c) linearly amplifying the tag-template conjugates; (d) generating a plurality of sequence reads from the linearly amplified tag-template conjugates; and (e) determining a nucleotide sequence of each of the nucleic acids based on the frequencies, or numbers, of each type of nucleotide at each nucleotide position of each plurality of sequence reads having identical sequence tags.
A method for sequencing nucleic acids comprising:
A method for determining a nucleotide sequence of a rare nucleic acid, the method comprising the steps of:
A method for determining a nucleotide sequence of a rare nucleic acid, the method comprising the steps of:
Dependencies are inferred from the claim text.