Microorganisms engineered to use unconventional sources of nitrogen
AssigneeNovogy, Inc.
InventorsColin R. South, Arthur J. Shaw, IV
Life of this patent
Estimating the term…
Abstract
Disclosed are genetically engineered organisms, such as yeast and bacteria, that have the ability to metabolize atypical nitrogen sources, such as melamine and cyanamide. Fermentation methods using the genetically engineered organisms are also described. The methods of the invention are robust processes for the industrial bioproduction of a variety of compounds, including commodities, fine chemicals, and pharmaceuticals.
Claims19
The genetically engineered organism of claim 2, wherein the nucleic acid molecule comprises any one of the sequences set forth in SEQ ID NOS:1-15, 17-21, 23, 25, 27, 29, 31, 33, 37, and 45-57.
A genetically engineered organism, wherein the genetically engineered organism has been transformed by a nucleic acid molecule;
- the nucleic acid molecule comprises a non-native gene;
- and the non-native gene encodes for a non-native enzyme selected from the group consisting of allophanate hydrolase, biuret amidohydrolase, cyanuric acid amidohydrolase, guanine deaminase, ammeline hydrolase, ammelide hydrolyase, melamine deaminase, isopropylammelide isopropylaminohydrolase, cyanamide hydratase, urease, and urea carboxylase.
9-11. (canceled)
14-15. (canceled)
17-19. (canceled)
21-24. (canceled)
A recombinant vector comprising a gene operably linked to a promoter, wherein the gene encodes an enzyme;
- and the enzyme is allophanate hydrolase, biuret amidohydrolase, cyanuric acid amidohydrolase, guanine deaminase, melamine deaminase, isopropylammelide isopropylaminohydrolase, cyanamide hydratase, urease, or urea carboxylase.
Dependencies are inferred from the claim text.