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US 2016/0115216 A1Published application
Method for Making Mature Insulin Polypeptides
OwnerFrantisek Hubalek, Annette F. Pettersson +2inventors
InventorsFrantisek Hubalek, Annette F. Pettersson, Thomas B. Kjeldsen, Asser S. Andersen
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Abstract
This invention relates to an improved method for making mature human insulin or an analogue thereof by cultivating fungi cell comprising a DNA sequence encoding a precursor for human insulin or an analogue thereof, which precursor comprises a small connecting peptide.
Claims15
An insulin precursor comprising the sequence Z-B-X-Y-A, wherein
- Z is an optional extension sequence,
- B is the B-chain of human insulin or an analogue thereof,
- X is a sequence selected from the group consisting of X1M, EA, AE, AD, DA, and AP, where X1 is a sequence comprising from 1 to 3 amino acid residues,
- Y is K or R, and
- A is the A-chain of human insulin or an analogue thereof.
2 more elements
Dependencies are inferred from the claim text.