Methods and systems for collecting mononuclear cells
AssigneeFenwal, Inc.
InventorKatherine N. Radwanski
Life of this patent
Estimating the term…
Abstract
A method for obtaining MNCs is set forth. The method includes: separating mononuclear cells from a biological fluid that includes red blood cells, plasma and platelets and collecting a targeted number of mononuclear cells in a suspension including plasma and residual red blood cells and platelets; concentrating the separated mononuclear cells; removing plasma from the concentrated mononuclear cells until the amount of residual plasma remaining with the concentrated mononuclear cells reaches a pre-determined volume; and adding a crystalloid solution to the concentrated mononuclear cells. Related apparatus and resultant MNC products are also disclosed.
Claims22
A method for obtaining mononuclear cells and preparing the mononuclear cells for photopheresis with a separation device comprising:
- a) separating mononuclear cells from a biological fluid that includes red blood cells, plasma and platelets;
- b) collecting a targeted number of mononuclear cells in a suspension including plasma and residual red blood cells and platelets;
- c) concentrating said separated mononuclear cells;
- d) removing plasma from the concentrated mononuclear cells until the amount of residual plasma remaining with the concentrated mononuclear cells reaches a pre-determined volume;
- and
- e) adding a crystalloid solution to the concentrated mononuclear cells.
A system for the collection of mononuclear cells comprising:
- a) a reusable hardware apparatus, said apparatus including a separation device and a programmable microprocessor driven controller including instructions for processing a biological fluid;
- b) a disposable processing circuit associated with said apparatus, said circuit including a processing chamber for receiving a biological fluid and one or more containers for collecting mononuclear cells;
- c) wherein said controller is programmed to:
- i. separate mononuclear cells from a biological fluid that includes red blood cells, plasma and platelets;
- ii. concentrate said obtained mononuclear cells and remove plasma from the concentrated mononuclear cells until the amount of residual plasma remaining with the concentrated mononuclear cells reaches a pre-determined volume;
- and
- iii. combine said obtained platelets with a crystalloid solution.
A mononuclear cell product comprising mononuclear cells, plasma, crystalloid solution and residual red blood cells and platelets having a total volume of less than or equal to (≦) 200 mL and a hematocrit of less than or equal to (≦) 2%, wherein the volume % of plasma is less than or equal to (≦) 25% and the volume of residual plasma remaining with said concentrated mononuclear cells is less than or equal to (≦) 50 ml.
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Dependencies are inferred from the claim text.