Lipofilling with ex-vivo expanded adipose tissue-derived stem cells for cosmetic breast filling or for facial filling and/or rejuvenation

A composition comprising ex-vivo expanded primary culture (P0) adipose tissue-derived stem cells (ASCs) mixed with harvested fat tissue at a ratio of at least 20×106-20×107 ASCs/mL fat, wherein said ex-vivo expanded primary culture (P0) adipose tissue-derived stem cells (ASCs) have been cultured in a growth medium consisting of Dulbecco's modified Eagle's medium (DMEM) or Alpha minimal essential medium (α-MEM), 1-5% penicillin-streptomycin, 1-5 IU/mL preservative-free heparin and 2-20% pooled Human Platelet Lysate (pHPL) and have been harvested in the primary passage (P0).

A cosmetic method of breast filling wherein ex-vivo expanded primary culture (P0) adipose tissue-derived stem cells (ASCs) are mixed with harvested fat tissue at a ratio of at least 20×106-20×107 ASCs/mL fat, wherein said primary culture (P0) adipose tissue-derived stem cells (ASCs) have been cultured in a growth medium consisting of Dulbecco's modified Eagle's medium (DMEM) or Alpha minimal essential medium (α-MEM), 1-5% penicillin-streptomycin, 1-5 IU/mL preservative-free heparin and 2-20% pooled Human Platelet Lysate (pHPL) and have been harvested in the primary passage (P0) and wherein the fat is injected as aliquots or as strings with a long needle horizontally (parallel to the body) by inserting the needle from several points around the areola margin and at several points at the inframammary fold in variable directions and planes to achieve an even and natural appearing distribution of the graft.

A cosmetic method of facial filling wherein ex-vivo expanded primary culture (P0) adipose tissue-derived stem cells (ASCs) are mixed with harvested fat tissue at a ratio of at least 20×106-20×107 ASCs/mL fat, wherein said primary culture (P0) adipose tissue-derived stem cells (ASCs) have been cultured in a growth medium consisting of Dulbecco's modified Eagle's medium (DMEM) or Alpha minimal essential medium (α-MEM), 1-5% penicillin-streptomycin, 1-5 IU/mL preservative-free heparin and 2-20% pooled Human Platelet Lysate (pHPL) and have been harvested in the primary passage (P0) and wherein the fat grafts are injected as aliquots or as strings with a long needle horizontally (parallel to the surface) to avoid damaging structures outside the target area and the needle is inserted from several points and in variable directions and planes to achieve an even and natural appearing distribution of the graft.

A cosmetic method of facial filling wherein ex-vivo expanded primary culture (P0) adipose tissue-derived stem cells (ASCs) are mixed with harvested fat tissue at a ratio of at least 20×106-20×107 ASCs/mL fat, wherein said primary culture (P0) adipose tissue-derived stem cells (ASCs) have been cultured in a growth medium consisting of Dulbecco's modified Eagle's medium (DMEM) or Alpha minimal essential medium (α-MEM), 1-5% penicillin-streptomycin, 1-5 IU/mL preservative-free heparin and 2-20% pooled Human Platelet Lysate (pHPL) and have been harvested in the primary passage (P0) and wherein the ASCs are injected in the dermis with a thin sharp needle and evenly distributed in the target area and the incision and injection sites are sutured and postoperative compression garments are applied to the donor sites and in some cases also to the recipient sites.

A cosmetic method of introducing an agent to the skin wherein the agent comprises ex-vivo expanded primary culture (P0) adipose tissue-derived stem cells (ASCs) mixed with harvested fat tissue at a ratio of at least 20×106-20×107 ASCs/mL fat, wherein said ex-vivo expanded primary culture (P0) adipose tissue-derived stem cells (ASCs) have been cultured in a growth medium consisting of Dulbecco's modified Eagle's medium (DMEM) or Alpha minimal essential medium (α-MEM), 1-5% penicillin-streptomycin, 1-5 IU/mL preservative-free heparin and 2-20% pooled Human Platelet Lysate (pHPL) and have been harvested in the primary passage (P0).