Systems And Methods For Model-Based qPCR

A system for determining a cycle threshold for a PCR amplification curve, the system comprising:

• a processor;
• and
• a memory storing instructions executable by the processor, the instructions comprising instructions for:
• receiving a data set for a plurality of biological samples for a PCR amplification reaction, wherein the data set includes a plurality of amplification curves, each amplification curve associated with a biological sample of the plurality of biological samples;
• and
• displaying, on a computer display, a modeled amplification curve and a cycle threshold value for each biological sample, wherein each cycle threshold value is determined by a processor based on a complementary relationship of a modeled efficiency curve to the modeled amplification curve for each sample, wherein each modeled amplification curve is determined and generated by a processor using the associated modeled efficiency curve on a cycle-by-cycle basis, wherein each modeled efficiency curve is determined and generated by a processor using a nonlinear optimization.