Medical prognosis and prediction of treatment response using multiple cellular signalling pathway activities

A method comprising:

• inferring activity of two or more cellular signaling pathways in a tissue and/or cells and/or a body fluid of a subject based at least on the expression levels (20) of one or more target gene(s) of the cellular signaling pathways measured in an extracted sample of the tissue and/or the cells and/or the body fluid of the subject, and
• determining a risk score that indicates a risk that a clinical event will occur within a certain period of time, wherein the risk score is based at least in part on a combination of the inferred activities,
• wherein the cellular signaling pathways comprise a Wnt pathway, an ER pathway, an HH pathway, and/or an AR pathway,
• wherein the cellular signaling pathways comprise the ER pathway, the Wnt pathway, and the HH pathway, and wherein the risk score is defined such that the indicated risk that the clinical event will occur within the certain period of time decreases with an increasing PER and increases with an increasing max(PWnt, PHH),
• wherein PER, PWnt, and PHH denote the inferred activity of the ER pathway, the Wnt pathway, and the HH pathway, respectively.