Optical scanning systems for in situ genetic analysis
AssigneeIllumina, Inc.
InventorWenyi Feng
Life of this patent
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Abstract
Presented herein are systems and methods for performing sequencing, including fluorescence in situ sequencing. In one embodiment, a confocal time delay and integration (TDI) line scan imaging system may include various pinhole and/or slit aperture mechanisms in front of the image sensor. The system may also include structures with focusing strips on a substrate in contact with the tissue sample to be imaged. Alternatively, these strips may be cut into the tissue sample. The system may also include configurations and methods of placing a tissue sample inside a reaction chamber of a flow cell during the assembly of the flow cell and then performing chemistry operations on the tissue sample. The flow cells may use an open container for performing chemistry operations on the tissue sample.
Claims32
A confocal TDI line scan imaging system comprising:
- a light source aperture;
- an optical beam splitter;
- a lens;
- a TDI image sensor and
- a sensor aperture mechanism.
A method of processing a tissue sample comprising:
- providing a first substrate of a flow cell;
- placing a sample tissue on the first substrate;
- providing a second substrate and assembling the second substrate to the first substrate, wherein a reaction chamber is formed around the sample tissue;
- and
- performing chemistry operations on the sample tissue in the reaction chamber
Dependencies are inferred from the claim text.