Method of measuring lipoprotein's capacity to accept cholesterol
AssigneeSysmex Corporation
InventorsAmane HARADA, Katsuhiro MURAKAMI, Maria KIRIYAMA, Keiko YOSHIKAWA, Keiko MIWA
Life of this patent
Estimating the term…
Abstract
Provided is a method of measuring a lipoprotein's capacity to accept cholesterol including the steps of: bringing a lipoprotein in a sample into contact with labeled cholesterol to incorporate the labeled cholesterol into the lipoprotein; bringing the lipoprotein having the labeled cholesterol into contact with an antibody that binds to a lipoprotein to form a complex of the lipoprotein and the antibody; and measuring the label incorporated into the complex.
Claims20
A method of measuring a lipoprotein's capacity to accept cholesterol comprising the steps of:
- bringing a lipoprotein in a sample into contact with labeled cholesterol to incorporate the labeled cholesterol into the lipoprotein;
- bringing the lipoprotein having the labeled cholesterol into contact with an antibody that binds to a lipoprotein to form a complex of the lipoprotein and the antibody;
- and
- measuring the label generated from the complex.
A method of measuring a lipoprotein's capacity to accept cholesterol comprising the steps of:
- bringing a high-density lipoprotein in a sample into contact with labeled cholesterol to incorporate the labeled cholesterol into the high-density lipoprotein;
- bringing the high-density lipoprotein having the labeled cholesterol into contact with an antibody that binds to a high-density lipoprotein to form a complex of the high-density lipoprotein and the antibody;
- and
- measuring the label incorporated into the complex,
- wherein the step of incorporating the labeled cholesterol into the high-densitylipoprotein, the step of forming the complex, and the step of measuring the label are performed in a cell-free system.
A method of measuring a lipoprotein's capacity to accept cholesterol comprising the steps of:
- bringing a high-density lipoprotein in a sample into contact with labeled cholesterol to incorporate the labeled cholesterol into the high-density lipoprotein;
- bringing the high-density lipoprotein having the labeled cholesterol into contact with an anti-ApoAI antibody which is immobilized on a solid phase to form a complex of the high-density lipoprotein and the anti-ApoAI antibody on the solid phase;
- and
- measuring the label incorporated into the complex,
- wherein the step of incorporating the labeled cholesterol into the high-densitylipoprotein, the step of forming the complex, and the step of measuring the label are performed in a cell-free system.
Dependencies are inferred from the claim text.