Tsh antibodies for point-of-care immunoassay formats
AssigneeAbbott Point of Care Inc.
InventorsDan WANG, Gordon Bruce Collier, Joseph P. Skinner, Qiaoqiao Ruan, Sergey Y. Tetin
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Abstract
The invention relates to antibody characteristics used to design a whole blood Point of Care Thyroid Stimulating Hormone (TSH) immunoassay using an ELISA sandwich assay lacking one or more wash steps between the antigen capture, detection antibody addition and substrate introduction steps. This invention exhibits low cross reactivity with biologically similar interfering cross reacting species, such as Follicle Stimulating Hormone (FSH), Luteinizing Hormone (LH) and Chorionic Gonadotropin (CG).
Claims20
A method for performing a thyroid stimulating hormone (TSH) immunoassay, the method comprising the steps of:
- (a) inserting a fluid sample into a device comprising an immunosensor in a conduit, said immunosensor having a capture antibody specific for TSH immobilized thereon;
- (b) dissolving a signal antibody into said fluid sample, wherein the capture antibody and the signal antibody each have a dissociation constant (Kd) for TSH of between 0.08 and 0.1 nM;
- (c) forming a sandwich complex on said immunosensor, said complex comprising said capture antibody, TSH, and said signal antibody;
- and
- (d) detecting a signal associated with said complexed signal antibody.
A method for performing a thyroid stimulating hormone (TSH) immunoassay, the method comprising the steps of:
- (a) inserting a fluid sample into a device comprising an immunosensor in a conduit, said immunosensor having a capture antibody specific for TSH immobilized thereon;
- (b) dissolving a signal antibody into said fluid sample, wherein the capture antibody and the signal antibody each have a dissociation constant (Kd) for TSH of between 0.04 and 0.1 nM;
- (c) forming a sandwich complex on said immunosensor, said complex comprising said capture antibody, TSH, and said signal antibody;
- and
- (d) detecting a signal associated with said complexed signal antibody.
Dependencies are inferred from the claim text.