Detection of gluten-specific t-cells
AssigneeOslo Universitetssykehus Hf
InventorsLudvig Sollid, Shuo-wang Qiao, Asbjorn Christophersen, Knut E.A. Lundin
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Abstract
The present invention relates to compositions and methods for visualizing disease-specific T-cells. In particular, the present invention relates to compositions and methods for use in the diagnosis, monitoring of progression, monitoring of response to therapy, and selection of patients for therapy of autoimmune diseases characterized by selective expansion of disease-specific effector memory T-cells.
Claims23
A method of identifying a disease-specific T-cell response, comprising:
- a) contacting a blood sample from a subject with a multimeric polypeptide comprising a plurality of disease epitope peptides;
- b) identifying the presence of T-cells that bind to said disease epitope peptides to generate disease-specific T-cells;
- c) separating said CD4+ T-cells into Naïve (TN), central memory (TCM) and effector memory (TEM) CD4+ T− cells and quantifying said TN, TCM, and TEM CD4+ T-cells;
- d) determining whether the ratio of CD4+ TEM− cells to CD4+ TN cells in said sample is above a pre-determined threshold level.
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A method of identifying a disease-specific T-cell response, comprising:
- a) contacting a blood sample from a subject with a multimeric polypeptide comprising a plurality of disease epitope peptides, wherein said disease epitope peptides include at least is the DQ2.5 and DQ2.2 epitopes;
- b) identifying the presence of T-cells that bind to said disease epitope peptides to generate disease-specific T-cells;
- c) separating said CD4+ T-cells into Naïve (TN), central memory (TCM) and effector memory (TEM) CD4+ T-cells and quantifying said TN, TCM, and TEM CD4+ T-cells;
- and
- d) characterizing said subject as having said disease when the ratio of CD4+TEM− cells to CD4+ TN cells is one or greater in said sample.
A method of identifying a disease-specific T-cell response, comprising:
- a) contacting a blood sample from a subject with a multimeric polypeptide comprising a plurality of disease epitope peptides;
- b) identifying the presence of T-cells that bind to said disease epitope peptides to generate disease-specific T-cells;
- c) separating said CD4+ T-cells into Naïve (TN), central memory (TCM) and effector memory (TEM) CD4+ T-cells and quantifying said TN, TCM, and TEM CD4+ T-cells;
- d) identifying the level of TEM cells positive for integrin-β7 expression;
- and
- d) determining whether the ratio of CD4+ TEM− cells to CD4+ TN cells and/or the level of integrin-β7 expression in said sample is/are above predetermined threshold level(s).
Dependencies are inferred from the claim text.