Apparatus for detecting bio materials, method of fabricating the apparatus and method of detecting bio materials using the apparatus

An apparatus for detecting bio materials, comprising:

• a biosensor comprising a reaction part, a sensor part and a calculation part;
• and
• a stand configured to fix and support the biosensor,
• wherein the reaction part comprises:
• a microtube comprising a first end and a second end;
• a filter disposed adjacent to the first end and configured to filter a body fluid provided from the first end;
• a first reaction region disposed between the filter and the second end in the microtube, the first reaction region comprising bio material detecting materials in a freeze dried state configured to make a specific binding with a bio material in the body fluid filtered;
• and
• a second reaction region disposed between the first reaction region and the second end, the second reaction region comprising bio material capturing materials fixed to an inner surface of the microtube, the bio material capturing materials being configured to make a specific binding with the bio material.

A method of fabricating an apparatus for detecting bio materials, the method comprising:

• preparing a hollow microtube having a first end and a second end;
• fixing bio material capturing materials to an inner surface of the microtube adjacent to the first end of the microtube so as to have a directional property;
• freeze drying the bio material detecting materials between a region where the bio material capturing materials are fixed and the second end;
• and
• disposing a filter combined with blood cell capturing antibodies between a region where the bio material detecting materials are freeze dried and the second end.

A method of detecting bio materials, the method comprising:

• injecting a body fluid including bio materials into a first end of a microtube;
• passing the body fluid through a filter in the microtube for filtration;
• combining the bio materials in the body fluid with bio material detecting materials, respectively;
• combining the bio materials combined with the bio material detecting materials, respectively, with bio material capturing materials, respectively;
• and
• perceiving the presence and the amount of the bio materials, the bio materials being fixed by the bio material capturing materials and being combined with the bio material detecting materials.