Methods and materials for assessing pluripotency of stem cell populations
AssigneeMayo Foundation for Medical Education and Research
InventorsTimothy J. Nelson, Alyson J. Smith, Frank J. Secreto
Life of this patent
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Abstract
This document provides methods and materials for assessing the pluripotency of stem cell populations. For example, methods and materials for using a DNA damaging agent (e.g., etoposide) to assess the pluripotency of a population of stem cells (e.g., a population of human induced pluripotent stem cells) are provided.
Claims9
A method for releasing a stem cell population for clinical diagnostic or therapeutic use, wherein said method comprises:
- (a) obtaining samples of cells from said stem cell population,
- (b) contacting said samples with different concentrations of a DNA damaging agent,
- (c) performing fluorescence-activated cell sorting using said samples to determine that the concentration of said DNA damaging agent that results in about 50 percent of the cells within one of said samples to undergo apoptosis is less than about 300 nM, and
- (d) releasing said stem cell population for clinical diagnostic or therapeutic use.
A method for releasing a stem cell population for clinical diagnostic or therapeutic use, wherein said method comprises:
- (a) contacting samples of cells from said stem cell population with different concentrations of a DNA damaging agent,
- (b) performing fluorescence-activated cell sorting using said samples to determine that the concentration of said DNA damaging agent that results in about 50 percent of the cells within one of said samples to undergo apoptosis is less than about 300 nM, and
- (c) releasing said stem cell population for clinical diagnostic or therapeutic use.
A method for assessing the pluripotency of a stem cell population, wherein said method comprises:
- (a) obtaining samples of cells from said stem cell population,
- (b) contacting said samples with different concentrations of a DNA damaging agent, and
- (c) performing fluorescence-activated cell sorting using said samples to determine the concentration of said DNA damaging agent that results in about 50 percent of the cells within one of said samples to undergo apoptosis, wherein said stem cell population exhibits acceptable pluripotency when said concentration is less than 300 nM, and wherein said stem cell population exhibits unacceptable pluripotency when said concentration is greater than 300 nM.
Dependencies are inferred from the claim text.