Back to results
US 2016/0109368 A1Published application
Nucleic acid analyzer and nucleic acid analysis method using same
AssigneeHitachi High-Technologies Corporation
InventorsMichiru FUJIOKA, Motohiro YAMAZAKI, Toru YOKOYAMA
Life of this patent
Estimating the term…
Abstract
Provided is a nucleic acid analyzer, which does not require manual processes by a highly trained operator such as a researcher and is easy to use, small-sized, capable of accepting multiple samples, and performs speedy analysis, and a nucleic acid analysis method using the analyzer. The analyzer and method perform detection in a plurality of exposure times, provide a program for determining a threshold for signal detection, and determine whether a faint signal peak is a false signal peak.
Claims14
A nucleic acid analysis method comprising the steps of:
- irradiating, with light, an analysis sample including a plurality of DNA fragments;
- detecting fluorescence, excited from the analysis sample, corresponding to the DNA fragments with an imaging element within a predetermined detection time;
- setting a lower limit of fluorescence intensity required for analysis of the analysis sample within a detectable range of fluorescence intensity of the imaging element;
- acquiring fluorescence intensity for each of the DNA fragments based on the lower limit;
- detecting a peak of the fluorescence intensity acquired for each of the DNA fragments and determining time information corresponding to the peak;
- and
- displaying correspondence of the fluorescence intensity to the time information for each of the DNA fragments,
- wherein the display displays at least one or more peaks including a first peak having first fluorescence intensity and a second peak having fluorescence intensity weaker than the first fluorescence intensity, and
- the lower limit is adjusted with a predetermined value and fluorescence intensity, where measurement is made using the adjusted lower limit as the lower limit of the imaging element, is reset as fluorescence intensity of at least one or more peaks including the second peak, thereby determining whether at least one or more peaks including the second peak are false signal peaks.
6 more elements
A nucleic acid analyzer comprising:
- a light irradiating unit configured to irradiate, with light, an analysis sample including a plurality of DNA fragments;
- a detecting unit configured to detect fluorescence intensity, excited from the analysis sample, corresponding to the DNA fragments with an imaging element within a predetermined detection time;
- a storage configured to store a lower limit of fluorescence intensity required for analysis of the analysis sample within a detectable range of fluorescence intensity of the imaging element;
- an arithmetic control unit configured to control the respective units and to perform arithmetic processing;
- and
- a display unit configured to display a result from the arithmetic control unit,
- wherein the detecting unit acquires fluorescence intensity for each of the DNA fragments based on the lower limit,
- the arithmetic control unit detects a peak of the fluorescence intensity acquired for each of the DNA fragments and determines time information corresponding to the peak,
- the display unit displays correspondence of the fluorescence intensity to the time information for each of the DNA fragments, the display unit displays at least one or more peaks including a first peak having first fluorescence intensity and a second peak having fluorescence intensity weaker than the first fluorescence intensity, and
- the arithmetic control unit adjusts the lower limit with a predetermined value, resets fluorescence intensity, where measurement is made using the adjusted lower limit as the lower limit of the imaging element, as fluorescence intensity of at least one or more peaks including the second peak, and thereby determines whether at least one or more peaks including the second peak are false signal peaks.
7 more elements
Dependencies are inferred from the claim text.