Automated isolation and chemical reaction(s) of nucleic acids
AssigneeBiochain Institute, Inc.
InventorsXiaoliang HAN, James Jianming WANG, Tong LU
Life of this patent
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Abstract
The present teachings relate to methods, kits and devices for performing automated sequential nucleic acid isolation and conversion/purification in a single closed system. In various embodiments, the present teaching enable a user to (i) load a device with test samples, reagents and consumables; (ii) select or program the device for the desired nucleic acid isolation and subsequent chemical treatment and/or conversion reaction(s) without further user intervention; and recovering the isolated and treated and/or converted nucleic acid at the conclusion of the program once the device is activated.
Claims20
A method for performing sequential nucleic acid isolation and conversion/purification reaction(s) in a single closed system, wherein an isolated nucleic acid is prepared for modification detection, comprising:
- a. reacting the nucleic acid-containing sample with an extraction reagent, wherein an isolated nucleic acid is obtained from the sample, and
- b. reacting said isolated nucleic acid with a conversion reagent .wherein the said nucleic acid having the modification is prepared for further detection.
A method for performing sequential nucleic acid isolation and bisulfite conversion reaction in a single closed automated system wherein an isolated nucleic acid is prepared for modification detection, comprising:
- a. introducing a nucleic acid-containing sample into a reaction tube, wherein said reaction tube is within a device for performing sequential nucleic acid isolation and conversion/purification in a single closed system;
- b. reacting the nucleic acid-containing sample with an extraction reagent;
- c. binding the extracted nucleic acid to magnetic beads subsequently added after the extraction reaction, wherein an isolated nucleic acid is obtained from the sample;
- d. reacting said isolated nucleic acid with a bisulfite conversion reagent followed by an optional desulfonation reagent, and
- e. binding the bisulfite-converted nucleic acid to magnetic beads subsequently added after the conversion reaction, wherein said nucleic acid is obtained for further modification detection.
A device for performing sequential nucleic acid isolation and conversion/purification in a single closed device comprising:
- a. at least one sealed cartridge containing a plurality of reagents wherein the reagents are purification reagents and conversion reagents,
- b. a heating unit capable of contacting a plurality of reaction tubes, and
- c. a magnet configured to the reaction tubes.
Dependencies are inferred from the claim text.