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AssigneeBattelle Energy Alliance, LLC
InventorsDavid Neil Thompson, Emily DeCrescenzo Henriksen, David William Reed, Jill Renee Jensen
Estimating the term…
Methods for determining thermophilic enzymatic activity include heating a substrate solution in a plurality of closed volumes to a predetermined reaction temperature. Without opening the closed volumes, at least one enzyme is added, substantially simultaneously, to the closed volumes. At the predetermined reaction temperature, the closed volumes are agitated and then the activity of the at least one enzyme is determined. The methods are conducive for characterizing enzymes of high-temperature reactions, with insoluble substrates, with substrates and enzymes that do not readily intermix, and with low volumes of substrate and enzyme. Systems for characterizing the enzymes are also disclosed.
A system for determining enzymatic activity, the system comprising:
A conductive structure for supporting and heating a plurality of reaction vessels during agitation, the conductive structure comprising a block of a conductive material defining therein a plurality of wells protruding into the block from a first surface of the block and comprising at least one engagement feature protruding into the block from a sidewall surface of the block.
A method for forming a conductive structure for supporting and heating a plurality of reaction vessels during agitation, the method comprising:
Dependencies are inferred from the claim text.