Semi-continuous culture methods
AssigneeMara Renewables Corporation
InventorsZhiyong Sun, Michael Milway, Kevin Berryman, Mercia Valentine, Roberto E. Armenta, Laura Purdue
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Abstract
Provided herein are methods of culturing a microorganism. The methods include providing a container comprising one or more microorganisms in a medium, which has a first carbon to nitrogen ratio; culturing the microorganisms until the culture reaches a threshold indicator; harvesting a portion of the culture while maintaining the majority of the culture in the container; and adding fresh medium comprising a second carbon to nitrogen ratio to the container with the majority of the culture comprising the microorganisms.
Claims37
A method of culturing a microorganism comprising the steps of
- (a) providing a container comprising one or more microorganisms in a medium comprising a first carbon to nitrogen ratio;
- (b) culturing the microorganisms until the culture reaches a threshold indicator;
- (c) harvesting a portion of the culture while maintaining the majority of the culture in the container;
- and
- (d) adding fresh medium comprising a second carbon to nitrogen ratio to the container comprising the majority of the culture comprising the microorganisms.
Dependencies are inferred from the claim text.