US 2016/0108442 A1 · MICROORGANISMS AND METHODS FOR PRODUCING (3R)-HYDROXYBUTYL (3R)-HYDROXYBUTYRATE

Abstract

Provided herein are non-naturally occurring microbial organisms having a pathway for production of (3R)-hydroxybutyl (3R)-hydroxybutyrate, wherein the organism can further include a (R)-1,3-butanediol pathway, a (3R)-hydroxybutyrate pathway, a (3R)-hydroxybutyryl-CoA pathway, an acetoacetate pathway, an acetoacetyl-CoA pathway, a (3R)-hydroxybutyl-ACP pathway, or an acetoacetyl-ACP pathway. Additionally provided are methods and processes for producing and isolating (3R)-hydroxybutyl (3R)-hydroxybutyrate using the microbial organisms, and various compositions having the (3R)-hydroxybutyl (3R)-hydroxybutyrate. Still further provided are methods of treating or preventing a disease, disorder or condition using the (3R)-hydroxybutyl (3R)-hydroxybutyrate produced by the microbial organisms of the invention.

Claims27

Dependent claims

A non-naturally occurring microbial organism, said microbial organism having a (3R)-hydroxybutyl (3R)-hydroxybutyrate pathway and comprising at least one exogenous nucleic acid encoding a (3R)-hydroxybutyl (3R)-hydroxybutyrate pathway enzyme expressed in a sufficient amount to produce (3R)-hydroxybutyl (3R)-hydroxybutyrate, wherein said (3R)-hydroxybutyl (3R)-hydroxybutyrate pathway comprises a pathway selected from:

(1) 1A;
(2) 1B;
(3) 1C and 1E;
(4) 1D and 1E;
(5) 1F;
and
(6) 1G and 1E,
wherein 1A is a (3R)-hydroxybutyl (3R)-hydroxybutyrate ester forming enzyme, wherein 1B is a (3R)-hydroxybutyryl-CoA:(R)-1,3-butanediol alcohol transferase, wherein 1C is a (3R)-hydroxybutyl 3-oxobutyrate ester forming enzyme, wherein 1D is an acetoacetyl-CoA:(R)-1,3-butanediol alcohol transferase, wherein 1E is a (3R)-hydroxybutyl 3-oxobutyrate reductase, wherein 1F is a (3R)-hydroxybutyryl-ACP:(R)-1,3-butanediol ester synthase, wherein 1G is an acetoacetyl-ACP:(R)-1,3-butanediol ester synthase.

5 more elements

A process for producing (3R)-hydroxybutyl (3R)-hydroxybutyrate comprising:

(a) culturing a non-naturally occurring microbial organism having an (R)-1,3-butanediol pathway under conditions and for a sufficient period of time to produce (R)-1,3-butanediol in culture medium;
(b) culturing a non-naturally occurring microbial organism having a (3R)-hydroxybutyrate pathway under conditions and for a sufficient period of time to produce (3R)-hydroxybutyrate in culture medium;
and
(c) reacting the (R)-1,3-butanediol produced in step (a) and the (3R)-hydroxybutyrate produced in step (b) with a chemical catalyst or an enzyme to produce (3R)-hydroxybutyl (3R)-hydroxybutyrate.

1 more element

Dependencies are inferred from the claim text.

Microorganisms and methods for producing (3r)-hydroxybutyl (3r)-hydroxybutyrate

Life of this patent

Microorganisms and methods for producing (3r)-hydroxybutyl (3r)-hydroxybutyrate

Life of this patent

Abstract

Claims27